FINDING NEEDLES IN THE GENOMIC HAYSTACK

Capture-Seq is the most comprehensive targeted genotyping system for discovering and characterizing the genome for:

  • Allele mining: Pre-breeding marker discovery
  • SNP genotyping & haplotyping
  • QTL mapping & candidate gene identification
  • Genetic fingerprinting
  • Polyploid genomic selection
  • Resistance gene sequencing (RenSeq)
  • Pan-genome construction & mapping

Any organism can be analyzed using Capture-Seq, and we offer a suite of validated, ready-made Capture-Seq panels for industry-wide standardization of genotyping data.

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TARGETED EXOME SEQUENCING

  • 1,000 – 450,000+ targets
  • Predesigned & custom panels available
  • Legacy data compatible
  • Short and long read options
  • Illumina®
  • PacBio®

TISSUE/DNA TO FASTQ DATA, SNPS AND MORE

  • Minimum 500 samples for DNA extraction
  • Up to 5-week turnaround times

REPEATABLE AND RELIABLE

  • Sequence the same regions every time
  • <1% missing target data

SUITABLE FOR COMMERCIAL AND RESEARCH AGRICULTURE

  • No minimum sample requirement
  • Minimal ascertainment bias

Capture-Seq vs Arrays & GBS

Genotyping SystemTargeted Genotyping DataData Interval per TargetDevelopment CostEase of Customization & AdaptationMissing Data %Ascertainment BiasRead Depth at Targets (Equivalent Sequencing)RepeatabilitySuitable for Novel Marker DiscoverySuitable for Haplotype REconstructionEase if Copy Number & Dosage DetectionAnalytical Complexity
Capture-SeqYes400-500bpLowHighLowLowPredictable & ControllableHighYesYesHighLow
Chip-Based ArraysYes1bpHighLowLowHighN/AHighNoNoLowLow
RADSeq/GBSNo90bpLowVery LowHighN/AOften LowLowYesYesLowHigh

Capture-Seq Technology

ANSWERING MULTIPLE QUESTIONS IN TANDEM

Capture-Seq flexibly targets SNPs, genes, and QTLs at the same time to produce sequence data at each region, instead of a single SNP, through the power of NGS. This enables Capture-Seq to provide greater genomic insights into breeding material and training populations, including the ability to phase alleles into haplotypes for more effective polyploid results.

EASY & ADAPTIVE

Capture-Seq panels are suitable for different breeding programs of the same or closely related species, ensuring comprehensive pre-breeding marker discovery with minimal risk of ascertainment bias and wasted resources.

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Capture-Seq Technology

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COMPLETE RESULTS THAT ARE COMPLETELY RELIABLE.

Restriction enzyme genotyping-by-sequencing (GBS), sometimes called RADseq, datasets are inconsistent and produce high levels of missing data, especially in polyploid plants, making agricultural applications more challenging or impossible.

Capture-Seq leads the genotyping industry in low missing data. This ensures consistent, effective applications of breeding data to improve genetic gains.

Capture-Seq Genotyping Results

SpeciesGenotyping Objective# Capture-Seq Targets# SNPs DetectedMissing data %
MaizeFine mapping resistance genes (R-genes)5,0004,6230.15
SugarcaneGenomic selection and genetic mapping10,00037,9760.1
Loblolly PineGenomic selection20,00067,5250.002
BlueberryGenomic selection and genetic mapping31,000205,0570.03

If your pre-breeding work is complete and you are planning to scale up, we can help.

Discover Flex-Seq® Ex-L for high-volume genotyping Learn about implementing genomics in breeding

DISCOVER MORE WAYS TO IMPROVE AGRICULTURE.

FLEX-SEQ® EX-L

Genomic selection, parentage testing

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WHOLE-GENOME SEQUENCING

Marker discovery, genome skimming

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GD-SEQ

Genotyping for repetitive genomes

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BIOINFORMATIC ANALYSIS

Experienced, customized data analysis

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