Flex-Seq® Ex-L Rapid-Genomics

A Genotyping League of Its Own

Flex-Seq^® Ex-L is the ultra-specific, ultra-efficient, ultra-high-throughput targeted genotyping solution for:

Genomic selection
Genome edit verification
Marker-assisted selection & backcrossing
Parentage testing
Assessing population genetics & structure
Traceability

Flex-Seq^® Ex-L is designed for routine, high-volume breeding, starting from tissue/DNA to genotyping results in as few as two weeks.

FAST

Up to 2-week turnaround time – tissue/DNA to SNPs
Custom marker panel available in as few as 8 weeks

FOCUSED

Up to 98% marker recovery
Up to 99% on-target sequencing
99% agreement between technical reps
98% agreement with array technology
Greater balance & specificity than multiplexed PCR

FLEXIBLE

Any species/organism
Genotype public, proprietary and novel markers
Legacy data compatible
Update Flex-Seq^® Ex-L panels by adding/removing markers for evolving needs
Low DNA input requirements

FLEX-SEQ^® EX-L AT A GLANCE

Flex-Seq^® Ex-L genotyping panels target up to 20,000 markers:

SNPs & haplotypes
Genes
Short Indels

Flex-Seq^® Ex-L uses multiplexed hybridization for cleaner, more effective results vs multiplexed PCR

High quality data = high quality selections for breeding.

More adaptive than genotyping arrays

Easily customized panels enable greater flexibility to genotype specific markers and legacy datasets
Flex-Seq^® Ex-L readily scales to any breeding volume

More reliable than GBS / RADseq

Confident, repeatable genotyping results (including polyploids)

More cost-effective than both

Industry-leading price points and efficiency at equivalent marker densities

Flex-Seq^® Ex-L Technology

View Full Diagram

Flex-Seq^® Ex-L Panel Performance

Summary metrics of genotyping results using a 5,000 marker panel. Data Uniformity displays the percentage of markers with >0.2x mean sequence depth (93% – 95%). Markers Recovered shows the percentage of markers (97% – 98%) with sufficient sequencing depth for accurate genotyping (i.e. 10X per haploid genome). Sequencing Reads on Target reflects the reaction specificity, with 99% of all aligned sequencing data mapping to the intended targets.

The combination of high marker recovery, data uniformity and reaction specificity ensures efficiency and overall completeness of genotyping results with as many as 5,000 markers.

Flex-Seq^® Ex-L Panel Performance
5,000 Markers

Flex-Seq^® Ex-L for Animal Breeding

Expand the power of genomics in your breeding stock

Use high-density genotype data (e.g. whole-genome sequencing, Capture-Seq) to identify the most informative markers in elite breeding lines.
Use Flex-Seq^® Ex-L to genotype the most informative markers in everything else.
- Develop breed-specific panels
- Genomic selection, parentage testing, traceability

Multiple tissue types accepted:

Ear, tail & fin clips
Hair
Blood or semen

Get Started Today Learn More About Flex-Seq® Ex-L

Flex-Seq^® Ex-L for Plant Breeding

Flex-Seq^® Ex-L is designed for high volume breeding programs and effective in complex genomes. Genotyping has never been easier for:

Field & Forage Crops
Vegetables
Fruits
Trees
Every other plant, including polyploids

Develop Flex-Seq^® Ex-L panels for multiple breeding zones and merge with high-density genotype data to implement genomic selection.

Using a new product doesn’t mean starting over – Flex-Seq^® Ex-L’s legacy compatibility ensures continued usage of past genotyping efforts. Multiple tissue types accepted: