Flex-Seq® Ex-L is the ultra-specific, ultra-efficient, ultra-high-throughput targeted genotyping solution for:
Flex-Seq® Ex-L is designed for routine, high-volume breeding, starting from tissue/DNA to genotyping results in as few as two weeks.
Flex-Seq® Ex-L genotyping panels target up to 20,000 markers:
Flex-Seq® Ex-L uses multiplexed hybridization for cleaner, more effective results vs multiplexed PCR
More adaptive than genotyping arrays
More reliable than GBS / RADseq
More cost-effective than both
Flex-Seq® Ex-L’s hybridization technology delivers superior targeted genotyping results at higher marker densities compared to multiplexed PCR. Ex-L probes flexibly bind to and amplify targeted genomic regions, providing greater ability to recover specific markers of interest vs chip-based arrays. By sequencing an interval of DNA instead of a single nucleotide, Flex-Seq® Ex-L can also characterize multiple nearby markers for enhanced haplotype phasing of complex genomes.
Summary metrics of genotyping results using a 5,000 marker panel. Data Uniformity displays the percentage of markers with >0.2x mean sequence depth (93% – 95%). Markers Recovered shows the percentage of markers (97% – 98%) with sufficient sequencing depth for accurate genotyping (i.e. 10X per haploid genome). Sequencing Reads on Target reflects the reaction specificity, with 99% of all aligned sequencing data mapping to the intended targets.
The combination of high marker recovery, data uniformity and reaction specificity ensures efficiency and overall completeness of genotyping results with as many as 5,000 markers.
Expand the power of genomics in your breeding stock
Multiple tissue types accepted:
Flex-Seq® Ex-L is designed for high volume breeding programs and effective in complex genomes. Genotyping has never been easier for:
Develop Flex-Seq® Ex-L panels for multiple breeding zones and merge with high-density genotype data to implement genomic selection.
Using a new product doesn’t mean starting over – Flex-Seq® Ex-L’s legacy compatibility ensures continued usage of past genotyping efforts. Multiple tissue types accepted:
Using Flex-Seq® Ex-L for genomic selection eliminates the need for phenotyping.